of ROS / RNS
At Enterosys, we developed an innovative method for the ultrasensitive detection ROS (Reactive Oxygen Species) and RNS (Reactive Nitrogen species) (NO and H2O2) in real time with amperometric probes, used to predict the antioxidant capacity of new skin treatment compounds.
This method allows:
- To validate the antioxidant impact in in vitro or in vivo models, short and long-term predictions (NMEs, natural extracts, formulations, etc.)
- To compare the antioxidant profile of your bioactive molecules to a reference compound
- To use our testing models, adapt them or develop tailor-made models
- We validate the antioxidant or non-toxic properties of new actives with sensitive and highly specific NO and H2O2 probes
- In vitro or in vivo models in the fields of pharmaceutical, dermo-cosmetic and nutraceutical
- In real-time, with continuous, direct and exquisitely sensitive measurements (from 0.2 nM to µM, accuracy 1-10 pA/nM, up to 10 measures/second)
- Validated against Western Blot and Amplex Red
- Performance documented in >20 peer-reviewed publications
- 3 times more information and 10 times higher sensitivity in half the time compared to all currently available tools
Our models & Capabilities
Existing, validated models
Out-of-the box models
Client-specific custom models
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The gut motility describes the inherent ability of the smooth muscle constituting the wall of the intestine to contract spontaneously and rhythmically. This phenomenon is mostly dependent of enteric nervous system, particularly the myenteric plexus.
The gastrointestinal tract, is divided into four distinct parts (duodenum, jejunum, ileum and colon). These four segments have distinctly different functions to perform and different patterns of motility, because of different roles (mixing, transport and absorption of intraluminal contents in the small intestine and reabsorption of water and elimination of indigestible food residues in colon).
Abnormal motility or abnormal sensitivity in any part of the gastrointestinal tract can cause characteristic symptoms and induce gastrointestinal motility disorders.
Enterosys relies on a highly qualified team and technical platform to evaluate the impact of your drug candidate on gut motility using isotonic sensors in ex vivo models
- Screening of potential compounds
- Identification of the targeted intestinal segments (duodenum, jejunum, ileum and colon)
- Characterization or consolidation of the therapeutic effects
- Characterization or consolidation of the mechanism of action involving the enteric nervous system
- Repositioning of your compound
intestinal sac model
The intestinal absorption is defined as the process of movement of a drug or a bioactive compound from the route of administration to the systemic circulation. The epithelial barrier selectively regulates transport from the lumen to the underlying tissue compartments. Ex vivo permeability assays using “everted intestinal sacs" are a rapid and sensitive method of measuring either overall intestinal integrity or comparative transport of a specific molecule, with the added advantage of intestinal site specificity. This technique is a method of assessing drug absorption, or to examine regional epithelial barrier / permeability dysfunction in animal models of gastrointestinal disease.
To characterize the biological effect of your compounds and its mechanism of action, Enterosys proposes a technical platform for functional studies and biomarkers exploration :
- Molecular biology
- Real-time PCR
- Cell viability, Enzyme and immune assays
Samples can be collected during the study and/or at the endpoint and delivered by the sponsors or analysed in-house.