The players : nitric oxide (NO) and hydrogen peroxide (H2O2)
Numerous scientific studies have demonstrated the key role of NO and H2O2 in regulating the skin’s balance. Released in small quantities by the cells of the skin barrier, these mediators are involved in processes that are essential for maintaining good skin health (cell renewal, respiration, healing, permeability, melanogenesis, cell communication, etc.). On the other hand, when the skin is subjected to stress (due to oxidizing agents, exacerbated inflammation, pollution…), the exacerbated release of NO and H2O2 leads to alterations in vital biological processes, which affect the skin’s equilibrium (lipid peroxidation, irreversible DNA damage, mitochondrial dysfunctions, breakdown of the barrier function…)
Our service offer
Enterosys has developed a solid expertise in real-time NO and H2O2 quantification. This technology makes it possible to quantify, in an absolute way, the release of these two biomarkers, in different biological models (primary cell cultures or cell lines in 2 or 3D, reconstructed epidermis or skin explants).
We propose different applications:
– Evaluation of the antioxidant capacities of your active ingredients: in vitro safety and efficacy studies (chemical molecules, natural extracts, formulations and more…)
– Optimization of a combination of active ingredients to identify the best antioxidant performance of product cocktails at the lowest concentration for each of them
– Comparative study of the effectiveness of active ingredients versus a standard ingredient or an ingredient already on the market
– Design and development of appropriate protocols for stress models (innovation opportunity) for the creation of new cosmetic lines with new competitive advantages and claims (erythema, pollution, infectious agents, atopic dermatitis, hormonal imbalance…)
Our added value
We use NO and H2O2, relevant biomarkers in skin physiopathology, to predict the effects of active ingredients upstream of the cosmetic product development process. The technology we use allows a direct and very accurate detection of these highly unstable and reactive redox entities. Moreover, all our analyses are performed in real time: this allows us to identify very early (ms) or longer (1h) effects, to perform several tests on the same biological sample, thus saving time and development costs.